منابع مشابه
Genome editing assessment using CRISPR Genome Analyzer (CRISPR-GA)
SUMMARY Clustered regularly interspaced short palindromic repeats (CRISPR)-based technologies have revolutionized human genome engineering and opened countless possibilities to basic science, synthetic biology and gene therapy. Albeit the enormous potential of these tools, their performance is far from perfect. It is essential to perform a posterior careful analysis of the gene editing experime...
متن کاملEfficient Mitochondrial Genome Editing by CRISPR/Cas9
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been widely used for nuclear DNA editing to generate mutations or correct specific disease alleles. Despite its flexible application, it has not been determined if CRISPR/Cas9, originally identified as a bacterial defense system against virus, can be targeted to mitochondria for mtDNA editing. Here, we show t...
متن کاملCRISPR/Cas9 in Genome Editing and Beyond.
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has ...
متن کاملNovel Genome Editing With CRISPR-Cas9
There are 9 Let7 family members present in keratinocytes, all acting as translational suppressors of the same target mRNAs. This redundancy implies that Let7 is crucial to cellular functioning, and it has indeed been found to act as a tumor suppressor, down regulating functions such as proliferation, cell motility, and de-differentiation. I hypothesized that increasing loss of Let7 loci would l...
متن کاملCRISPR genome editing: A general view
How to cite this article: Kumar RMR. CRISPR genome editing: A general view. J Genet Med Gene Ther. 2017; 1: 001-002. CRISPR technology has presented a path forward for genomic engineering and gene modi ication. The framework for the use of CRISPR technology to manipulate the human genome is of great interest and the form of its development and application has excited the researchers and biotech...
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ژورنال
عنوان ژورنال: Science-Business eXchange
سال: 2013
ISSN: 1945-3477
DOI: 10.1038/scibx.2013.77